Optical tweezers — from calibration to applications: a tutorial

Optical Elastography: From Theory to Clinical Applications

OPTiCAL TwEEzERS CALibRATiON AND bACTERiAL ADHESiON

EXECUTiVE SUMMARY In this project, we will be measuring the forces of the binding of bacteria. We will be working with Lactobacillus acidophilus NCFM, Lactobacillus galli­ narum 33199, Lactobacillus ruteri 23272, and Bifidobacterium. These bacteria are lactic acid bacteria and are used as probiotics. We also reviewed literature on the bacterial cell wall constituents and the properties of adhes...

Absolute calibration of optical tweezers including aberrations

R. S. Dutra, 2, 3 N. B. Viana, 3 P. A. Maia Neto, 3 and H. M. Nussenzveig 3 Instituto Federal de Educação, Ciência e Tecnologia, Rua Sebastião de Lacerda, Paracambi, RJ, 26600-000, Brasil LPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brasil Instituto de F́ısica, Universidade Federal do Rio de Janeiro, Caixa Postal 68528, Rio d...

TweezPal - Optical tweezers analysis and calibration software

Optical tweezers, a powerful tool for optical trapping, micromanipulation and force transduction, have in recent years become a standard technique commonly used in many research laboratories and university courses. Knowledge about the optical force acting on a trapped object can be gained only after a calibration procedure which has to be performed (by an expert) for each type of trapped object...

Combined holographic-mechanical optical tweezers: Construction, optimisation and calibration

We have combined a spatial light modulator (SLM) and galvanometer-mounted mirrors into an optical tweezers set-up. This provides great flexibility by allowing us to create an array of traps which can be moved in a smooth and fast way. To optimise the performance we investigated the effect of incidence angle on the SLM with respect to phase and intensity response. Although it is possible to use ...